RED BILBERRY (VACCINIUM VITIS-IDAED L.) MICROCLONAL REPRODUCTION

DOI: 10.35205/0558-1125-2022-77-100-107
UDC 634.738:581.143.6

N.O. YAREMKO, T.V. MEDVEDIEVA, K.M. UDOVYCHENKO, T.A. NATALCHUK, PhDs
Y.S. ZAPOLSKYI, Junior Research Worker
Institute of Horticulture, NAAS of Ukraine, 03027, Kyiv-27, 23, Sadova st., e-mail: nadiiayaremko@gmail.com

The agents and their concentrations (0.1 % HgCl2 solution and 1 % and 2 % “Lizoformin 3000” solutions) influence on the efficiency and the explants initiation was researched in order to red bilberry cultivate under the conditions. All of them appeared effective for obtaining explants however for the further regeneration the use of the 0.1 % solution was optimum (61-85 % of the regenerated explants depending on the exposition). Such explants amount was maximum when utilizing 70 % of alcohol (30 sec.) and 0.1 % of the HgCl2 solution (4 min). Three nutritive media were studied for the effiecent red bilberry reproduction, namely: Anderson, Zimmerman and Broome and McCown as well as the best concentration was selected which provided the highest reproduction coefficient of the cultivar Runo Bielawskie. For instance, a nutritive medium and concentration were selected for the maximum explants proliferation in the conditions in vitro (the medium WPM with the addition of 2-iP in the concentration 5 mg/l – the reproduction coefficient being 3.8). Besides, the investigated factors effect on the reproduction coefficient was established statistically, the medium composition influence being the greatest one – 91 %. The rest of the factors did not effect this index considerably. The possibility of the simultaneous rooting and adaptation directly under the conditions ex vitro was proved with an effectivity of approximately 90 % that makes it possible to save costs when growing plants commercially. The efficient methods of the cv Runo Bielawskie reproduction were elaborated for the first time in Ukraine. Those methods may be applied for the studied crop drawing in the industrial numbers.

Key words: red bilberry, explants, sterilization, reproduction, in vitro, in vivo, adaptation, cytokinin, 2-iP, WPM, medium, lizoformin, mercury chloride.

Список використаної літератури

1. Debnath S.C., Arigundam U. In vitro propagation strategies of medicinally important berry crop, lingonberry (Vaccinium vitis-idaea L.). Agronomy. 2020. Vol. 10. P. 744. DOI: https://doi.org/10.3390/agronomy10050744.
2. Paprštein F., Sedlák J. In vitro multiplication of lingonberry – Short Communication. Hort. Sci. (Prague). 2015. Vol. 42. P. 102-106.
3. Gustavsson B.A., Stanys V. Field performance of ‘Sanna’ Lingonberry derived by micropropagation vs. stem cuttings. HortScience. 2000. Vol. 35. № 4. P. 742-744.
4. Reed B.M., Abdelnour-Esquivel A. The use of zeatin to initiate in vitro cultures of Vaccinium species and cultivars. HortScience. 1991. Vol. 26. P. 1320-1322.
5. Ostrolúcka M.G., Libiaková G., Ondrušková E., Gajdošová A. In vitro propagation of Vaccinium species. Acta Universitias Latviensis. 2004. Vol. 676. P. 207-212.
6. George E.F. Plant propagation by tissue culture. Part 2. In Practice. Edington. UK : Exegetics Ltd., 1996.
7. Marcotrigiano M., McGlew S.P. A two-stage micropropagation system for cranberries. J. Am. SocHortic. Sci. 1991. Vol. 116. P. 911-916.
8. Debnath S.C., McRae K.B. In vitro culture of lingonberry (Vacciniumvitis-idaeal.) : the influence of cytokinins and media types on propagation. Small Fruits Rev. 2001. Vol. 1. P. 3-19.
9. Gajdošová A., Ostrolucká M.G., Libiaková G., Ondrušková E. Protocol for micropropagation of Vaccinium vitis-idaea L. In protocols for micropropagation of woody trees and fruits. S.M. Jain, H. Häggman (eds.). Berlin-Heidelberg-Germany-New York, Springer, 2007. P. 457-464.
10. Micropropagation under photoautotrophic conditions. In Micropropagation / T. Kozai, P.C. Debergh, R.H. Zimmerman (eds.). Dordrecht.  The Netherlands : Springer, 1991. P. 447-469.
11. Debnath S.C. Effects of carbon source and concentration on development of lingonberry (Vacciniumvitis-idaea L.) shoots cultivated in vitro from nodal explants. In Vitro Cell. Dev. Biol. Plant. 2005. Vol. 41. P. 145-150.
12. Liquid culture for efficient in vitro propagation of adventitious shoots in wild Vaccinium vitis-idaea ssp. minus (lingonberry) using temporary immersion and stationary bioreactors / U. Arigundam et al. SciHortic. 2020. Vol. 264. P. 1091-1099.
13. Pliszka K., Kawecki L. A new selection of lingonberries (Vaccinium vitis-idaea L.) in Poland. In Problems of rational utilization and reproduction of berry plants in boreal forests on the eve of the 21 century : Forest institute NASB. Gomel, 2000. P. 197-199.
14. Jaakola L., Tolvanen A., Laine K., Hohtola A. Effect of N6-isopentenyladenine concentration on growth initiation in vitro and rooting of bilberry and lingonberry microshoots. Plant cell, tissue and organ culture. 2001. Vol. 66. P. 73-77.
15. Zimmerman R.H., Broome O.C. Blueberry micropropagation. In Proceedings of the conference on nursery production of fruit plants through tissue culture-applications and feasibility : Beltsville, MD, USA, April 21-22, 1980. P. 1080.