Structural and spatial organization of the microbial biom of the rhiosphere of plants of eternal green such

DOI: 10.35205/0558-1125-2020-75-244-250

UDC 579.64:632.76


S.V. VERETIUK, Роst Graduate Student 

T.I. PATYKA, Doctor, Professor

N.V. PATYKA, Doctor, Professor, Corresponding Member of NAAS of Ukraine

M.V. BOIKO, Doctor

National University of Life and Environmental Sciences, 03041, Kyiv, 13, Heroi Oborony st., e-mail:

The features of the formation of the structural and spatial organization and biodiversity of the microbial complex of the rhizosphere of plants of the evergreen boxwood (Búxus sempervírens L.) in closed ground are considered. The abundance of the main taxonomic groups of microorganisms of the evergreen boxwood rhizosphere: bacteria, micromycetes, and actinomycetes has been estimated. According to the soil microorganisms population structure results, was established that the quantitative indicators of boxwood rhizospheric microorganisms  depends on the physiological state and age of the plants. It has been investigated that the rhizosphere of boxwood is characterized by an insignificant variety of bacterial and fungal microflora. It was shown that the number of bacteria in plant ontogenesis increases from 2.09 ± 0.37 to 4.90 ± 0.62 million/g of soil (10-year-old plants). There is a dynamics of an increase in the number of micromycetes by more than 10 times (2.16 ± 1.22 – 2 year olds; 20.24 ± 2.55 – 4 year olds; 25.94 ± 3.24 thousand CFU/g – 10 year olds) and actinomycetes – 2.4 times (1.87 ± 0.03 million CFU/g). When analyzing the qualitative composition and cultural and morphological properties of isolates of rhizosphere microorganisms of evergreen boxwood, dominant forms were identified that are assigned to representatives of the genera Bacillus, Pseudomonas, Rhizopus and Trichoderma. 

Key words: Búxus sempervírens L., rhizosphere, microbial biome, morphotypes, plant-microbial interaction.

Список використаної літератури

1.Гадзало Я.М., Патыка Н.В., Заришняк А.С. Агробиология ризосферы растений: монографія. К.: Аграрна наука, 2015. 386 с. 

2.Yang C.H., Crowley D.E. Rhizosphere microbial community structure in relation to root locftion plant iron nutritional status. Applied and environmental microbiology. 2000. Vol. 66. P. 345-351. 

3.Patyka N.V., Kaminski V.F. Agrobiology of Rhizosphere. Agricultural Scienceand Practice. 2014. Vol. 1, №3. P. 69-75. 

4.Formation rhizospheric trophic chains in soil agroecosystems / Patyka N.V.,  Bublik N.A., Patyka T.I., Кitaev O.I. Journal of Information Intelligence and knowledge. 2015. Vol. 7, № 3. P. 413-418. 

5.Методы почвенной микробиологии и биохимии /под ред. Звягинцева Д.Г. М. : МГУ, 1991. 304 с.

6.Полевой В.В.,  Саламатова Т.С. Физиология роста и развития растений. Л.: Изд-во Ленингр. ун-та, 1991. 240 с.