Use of «Hyacinth», and «Lisoformin-3000» for initiation of the aceptic culture of the sweet cherry rootstocks
DOI: 10.30250/0558-1125-2018-73-171-176
UDC 518.143.6:634.2:632.38
USE OF «HYACINTH», AND «LISOFORMIN-3000» FOR INITIATION OF THE ACEPTIC CULTURE OF THE SWEET CHERRY ROOTSTOCKS
V.Ya. RIABY, Ya.S. ZAPOLSKY, Post Graduate Assistants
S.O. VASIUTA, T.A. NATALCHUK, PhDs
Institute of Horticulture, NAAS of Ukraine, 03027, Kyiv-27, 23, Sadova st., e-mail: rya.vasya2014@ukr.net
The purpose of the researches was to the initiate aseptic culture of a number of sweet cherry clonal rootstocks, that is conditioned by the necessity of developing the elements of the mentioned crop clonal rootstocks microclonal propagation technology for their sanitation from the viral infection.
The experiments were conducted in the Department of Virology, Sanitation and Propagation of the Fruit and Small Fruit Crops of the Institute of Horticulture (NAAS). The objects were explants of the clonal rootstocks LC-52 (Cerasus vulgaris x Cerasus fruticosa), VSL-2 (Cerasus fruticosa x Cerasus lannesiana Carr), VC-13 [Cerasus vulgaris, Cerasus fruticosa] x [Cerasus maasckia], Studenykivska (Cerasus fruticosa x Cerasus vulgaris), Colt (Cerasus avium x Cerasus pseudocerasus), Gisela 5 and Gizela 6 (P. Cerasus x P. Canescens). The plant material (virus-free samples only) was taken during the bud growth and active bud growth from the plants tested on the virus-carriage by means of the immune ferment analysis.
In the process of the microclonal propagation there are four stages: the explants extraction and their introduction into the sterile culture; cultivation on the nutrient medium with the sequential subculturing up to obtaining microshoots (proliferation); rooting and adaptation to ex vitro conditions in the soil substrate.
The success of introducing plant material into the in vitro culture depends largely on the sterilization quality. In order to initiate the sterile culture the 70 % ethanol, commercial coagulant-water hyacinth-based purifier “Hyacinth” based on the aluminum hydrochloride (100 %), sublimate (0.1 % HgCl2) and were utilized as well as the 3 % solution of the preparation "Lizoformin-3000".
The effect of the sterilizers coagulants water purifiers (“Hyacinth” and “Lizoformin-3000”) on the sterilization and regeneration efficiency was investigated for the purpose of the sweet cherry rootstocks explants. These sterilizing agents as effective and low toxic appeared to be used successfully in order to initiate the aseptic culture.
Key words: rootstocks, in vitro, sanitation, explants, sterilization, sterilizing agents, exposition.
UDC 518.143.6:634.2:632.38
USE OF «HYACINTH», AND «LISOFORMIN-3000» FOR INITIATION OF THE ACEPTIC CULTURE OF THE SWEET CHERRY ROOTSTOCKS
V.Ya. RIABY, Ya.S. ZAPOLSKY, Post Graduate Assistants
S.O. VASIUTA, T.A. NATALCHUK, PhDs
Institute of Horticulture, NAAS of Ukraine, 03027, Kyiv-27, 23, Sadova st., e-mail: rya.vasya2014@ukr.net
The purpose of the researches was to the initiate aseptic culture of a number of sweet cherry clonal rootstocks, that is conditioned by the necessity of developing the elements of the mentioned crop clonal rootstocks microclonal propagation technology for their sanitation from the viral infection.
The experiments were conducted in the Department of Virology, Sanitation and Propagation of the Fruit and Small Fruit Crops of the Institute of Horticulture (NAAS). The objects were explants of the clonal rootstocks LC-52 (Cerasus vulgaris x Cerasus fruticosa), VSL-2 (Cerasus fruticosa x Cerasus lannesiana Carr), VC-13 [Cerasus vulgaris, Cerasus fruticosa] x [Cerasus maasckia], Studenykivska (Cerasus fruticosa x Cerasus vulgaris), Colt (Cerasus avium x Cerasus pseudocerasus), Gisela 5 and Gizela 6 (P. Cerasus x P. Canescens). The plant material (virus-free samples only) was taken during the bud growth and active bud growth from the plants tested on the virus-carriage by means of the immune ferment analysis.
In the process of the microclonal propagation there are four stages: the explants extraction and their introduction into the sterile culture; cultivation on the nutrient medium with the sequential subculturing up to obtaining microshoots (proliferation); rooting and adaptation to ex vitro conditions in the soil substrate.
The success of introducing plant material into the in vitro culture depends largely on the sterilization quality. In order to initiate the sterile culture the 70 % ethanol, commercial coagulant-water hyacinth-based purifier “Hyacinth” based on the aluminum hydrochloride (100 %), sublimate (0.1 % HgCl2) and were utilized as well as the 3 % solution of the preparation "Lizoformin-3000".
The effect of the sterilizers coagulants water purifiers (“Hyacinth” and “Lizoformin-3000”) on the sterilization and regeneration efficiency was investigated for the purpose of the sweet cherry rootstocks explants. These sterilizing agents as effective and low toxic appeared to be used successfully in order to initiate the aseptic culture.
Key words: rootstocks, in vitro, sanitation, explants, sterilization, sterilizing agents, exposition.
